Checkerboard dna–dna hybridization
WebJul 15, 2008 · The checkerboard DNA–DNA hybridization technique enables the simultaneous identification of distinct bacterial species in a large number of dental samples. The original technique employed digoxigenin-labeled whole genomic DNA probes which … WebThe results demonstrate that the checkerboard DNA-DNA hybridization technique can be employed to study the microbiota composition of mosquitoes and suggest that the technique is a straightforward technique that can be widely used for the characterization of the microbiota in mosquito populations. 4 PDF
Checkerboard dna–dna hybridization
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WebFeb 19, 2007 · DNA–DNA hybridization showed that the amplified genome of each species used either as target or as probe provided signals equivalent to controls and that amplification of a mixture of species provided signals comparable to those provided by … WebApr 2, 2024 · Presence of 40 subgingival species and the association among them in subgingival dental biofilm samples (n = 13,321) were analysed using checkerboard DNA-DNA hybridization and cluster analysis and community ordination techniques, respectively. The base of the pyramid represents the early colonizers, followed by the orange …
WebDNA-DNA hybridization generally refers to a molecular biology technique that measures the degree of genetic similarity between pools of DNA sequences. It is usually used to determine the genetic distance between two species. WebOct 1, 1994 · A method is introduced for hybridizing large numbers of DNA samples against large numbers of DNA probes on a single support membrane. Denatured DNA from up to 43 samples was fixed in separate lanes on a single membrane mounted in a Miniblotter 45.
WebOct 15, 2004 · Digoxigenin-labeled whole genomic probes to 40 common subgingival species were tested in a checkerboard hydridization format. Chemifluorescent signals resulting from the hybridization reactions were quantified using a Fluorimager and used … WebHybridiza-tion signals are detected using chemifluorescence procedures. Bacterial numbers are determined by analyzing hybridization signals using a Storm system. A genus-specific probe is used to assess the total number of streptococci and universal …
WebSubgingival biofilm samples were individually processed for the identification of 40 microbial species using the Checkerboard DNA–DNA hybridization technique, following the procedures previously described . The list of oral bacterial strains employed for the preparation of DNA probes and used in the present study is presented in Table 1. DNA ...
WebOct 15, 2004 · Digoxigenin-labeled whole genomic probes to 40 common subgingival species were tested in a checkerboard hydridization format. Chemifluorescent signals resulting from the hybridization reactions were quantified using a Fluorimager and used to evaluate sensitivity and specificity of the probes. shop structuresWebJun 11, 2024 · DNA-DNA hybridization A technique that is used to compare DNA from two different species, to locate or identify nucleotide sequences, and to establish the effective in vitro transfer of nuclear material to a new host. shopstudioc.comWebMar 31, 2024 · Ulvik IM, Saethre T, Bunaes DF, Lie SA, Enersen M, Leknes KN. A 12-month randomized controlled trial evaluating erythritol air-polishing versus curette/ultrasonic debridement of mandibular furcations in supportive periodontal therapy. BMC Oral Health. 2024 Jan 21;21(1):38. doi: 10.1186/s12903-021-01397-3. shop studioWebFeb 1, 2007 · Checkerboard DNA-DNA hybridization (CKB) is a technique that provides a simultaneous quantitative analysis of 40 microbial species against up to 28 mixed microbiota samples on a single membrane ... shop studio 41 napervilleWebCheckerboard DNA-DNA hybridization (CKB) is a technique that provides a simultaneous quantitative analysis of 40 microbial species against up to 28 mixed microbiota samples on a single membrane; using digoxigenin (DIG)-labeled, whole-genome DNA probes. shop studio game theory 71 discount codeWebJun 1, 2000 · The results of the checkerboard DNA-DNA hybridization analysis showed that 22 of the 42 DNA probes tested were reactive with 1 or more samples. The number of bacterial species in the root canal samples ranged from 1 to 17 (mean, 4.7). Seventeen of the 28 root canal samples were positive for at least 1 DNA probe. shops try to meet the diy fashionWeb"Checkerboard" DNA-DNA hybridization A method is introduced for hybridizing large numbers of DNA samples against large numbers of DNA probes on a single support membrane. Denatured DNA from up to 43 samples was fixed in separate lanes on a … shops tucson