Ctcf kda
WebCTCF has a predicted molecular weight of 84 kDa and CTCFL, 74 kDa. However, CTCF is known to migrate as a 130-kDa protein [39]. Since the transgenes are expressed as fusion proteins with FLAG tag... WebApr 6, 2012 · Here, we report that regulation of CTCF by de-SUMOylation is dependent upon hypoxic and oxidative stresses. We found that stimulation of human corneal …
Ctcf kda
Did you know?
Web~ 130 kDa Note: Poly(ADP-ribosyl)ated isoform, C-terminal truncation, or sumoylation will cause variable migration of CTCF protein on SDS–PAGE, which may present CTCF with … WebAnti-CTCF antibody [EPR7314(B)] - ChIP Grade (ab128873) at 1/10000 dilution + Mouse brain at 20 µg Secondary Goat Anti-Rabbit IgG H&L (HRP) at 1/100000 dilution …
WebCUT&RUN was performed on 500k K562 cells with either CTCF (0.125 µg) or IgG negative control (0.5 µg, EpiCypher 13-0042) antibodies using the CUTANA™ ChIC/CUT&RUN Kit v3.0 (EpiCypher 14-1048).Library preparation was performed with 5 ng of DNA (or the total amount recovered if less than 5 ng) using the CUTANA™ CUT&RUN Library Prep Kit … WebBPTF plays an important role in regulating nucleosome occupancy at nucleosome-free regions (NFRs) at sites occupied by the multivalent factors CTCF and cohesin via direct interaction with CTCF and the cohesin subunit SA2. In addition, BPTF also interacts with c-MYC and plays an essential role in its genomic distribution and biological activity.
WebThe Division of Data Processing is headed by a Chief of Data Processing and is responsible for the development, utilization and coordination of the Department's Data computer … WebPolyclonal Antibody for studying CTCF. Cited in 67 publications. Validated for WB, IP, IF, ChIP. Highly specific and rigorously validated in-house, CTCF Antibody (CST #2899) is …
WebDepartment of Children and Families. 505 Hudson Street. Hartford CT 06106. Phone Number: 860-550-6300. E-mail the Commissioner.
WebBackground Information. CTCF is a ubiquitous 11 zinc finger (ZF) protein with highly versatile functions. A transcriptional repressor, CTCF, binds to promoters of vertebrate c-myc gene. It also binds to the PLK and PIM1 promoters. CTCF may prevent the access of transcriptional activators to enhancers. greenfield indiana early votingWebDec 12, 2024 · Through binding of CTCF binding sites (CBS), CTCF mediates nuclear functions including DNA looping, regulation of gene expression, and RNA splicing. ... only two bands at 110 kDa and 130 kDa were ... fluorescent bulbs outlawedWebFeb 27, 2024 · The 82 kDa CTCF protein is highly conserved in most bilateral animals and is ubiq-uitously expressed in the nucleus across all tissue types. Between its N-terminal and. greenfield indiana fence permitWebMar 11, 2014 · CTCF creates boundaries between topologically associating domains in chromosomes and, within these domains, facilitates interactions between transcription … greenfield indiana clothing storesWebJul 10, 2024 · Silver-stained SDS PAGE gel of IP proteins showing chicken CTCF protein of approximately 140 kDa, and lamprey CTCF protein of >250 kDa. Detailed procedures of western blotting and ... greenfield indiana florist deliveryWebCCCTC-binding factor (CTCF) is an 82-kDa protein with 11 zinc fingers.1 It was first identified as a transcriptional repressor of the chicken c-myc gene, a regulatory gene that encodes the greenfield indiana food pantryfluorescent bulbs just glow