Topflash or fopflash

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Web恶性黑素瘤中SOX4的表达及其对Wntβcatenin信号途径作用研究,恶性黑素瘤,黑素瘤,黑素瘤与痣的区别图,黑素瘤能治好,catenin,黑素瘤早期图片,甲下黑素瘤,beta catenin,恶性肿瘤,恶性淋巴瘤 Web17. okt 2000 · The cells were transfected with 13.7 μg each of TOPFLASH or FOPFLASH reporter plasmids , human-Tcf-4, and β-catenin expression vectors using 18.3 μl of Lipofectamine (GIBCO/BRL). On the following day, the cells were replated at 5 × 10 5 into 6-well plates and incubated at 37°C for 8 h. The cells were washed in serum-free medium …

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WebThis 134bp fragment was gene synthesized and cloned into the ASP718 and XMA-1 sites of the vector. Note: This plasmid was published as M51 Super 8x FOPFlash, but the plasmid … WebBioVector质粒载体菌株细胞蛋白抗体基因保藏中心-NTCC典型培养物保藏中心，可提供数万种质粒载体、菌种、基因、细胞株和感受态细胞,蛋白,抗体,并可提供实验技术外包服务,如基因克隆、质粒构建、蛋白原核、真核表达及纯化、病毒包装、基因沉默RNAi等服务。 prodigy protein docking analyzer

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WebTopflash or Fopflash control was transfected into A7r5 cells along with expression constructs for β-catenin, IL2R-Fat1 IC , N-cadherin, and/or IL2R-E-cadherin IC . The … Web一般而言，topflash/fopflash是比较常用的检测β-catenin的系统，原理和所有的RLU一样，都是利用转录因子可以和特异性的靶位点结合进而启动下游的虫荧光素酶基因的表达。如 … Web14. aug 2015 · zt200 TOPFlash 载体 Millipore 信号通路报告载体系统： zt201 FOPFlash 载体 Millipore 信号通路报告载体系统： zt202 SuperTopFlash 载体 Millipore 信号通路报告载体系统： zt203 RNAi 系统载体 Ambion RNAi 系统载体. zt204 pSilencer1.0 载体 Ambion RNAi 系 … reinstall photoshop elements 2020

UBE2T promotes β‐catenin nuclear translocation in hepatocellular ...

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WebA, TOPFLASH analysis of 293T cells transfected with h-catenin, Cby, and/or TC1 (solid columns ). Applied DNAs are indicated for each lane. Experiments were repeated in … Web2. apr 2024 · wnt通路中，TOPflash/FOPflash有啥区别？答魔，致力于搭建生命科学领域的综合交流平台。凭借智能、高效、安全的数据挖掘处理 ... prodigy property management spokane waWeb17. sep 2024 · TOPFlash/FOPFlash reporter assay. To assess the influence of FOXQ1 levels on Wnt/β-catenin signaling activities, a TOPFlash/FOPFlash reporter assay was performed. Cells from the Foxq1-sh mBMSC, Foxq1-over mBMSC, lv3 mBMSC, and lv5 mBMSC groups and Foxq1-over+siAnxa2 mBMSCs were seeded on 96-well plates at a density of 4 × 10 3 … reinstall photoshop elements 2021

"WebFOPflash (mutant TCF binding sites) 21-169 Sigma-Aldrich FOPflash (mutant TCF binding sites) Transfection grade T cell factor (TCF) reporter plasmid containing 2 full & one incomplete copy of the Tcf binding site (mutated) followed by 3 copies in the reverse orientation. Serves as a negat More>> " - Topflash or fopflash

Topflash or fopflash

Induction of LEF1 by MYC activates the WNT pathway and …

Web我因为刚刚接触，用的是分别转染TOPflash和FOPflash，但遇到了问题。 2、FOPflash值不稳定，并不像有些文献里的那样，各组之间FOP值都不变，我的有时变化2-3倍。而且FOP值很高，同样量的TOP和FOP，FOP值远高于TOP，除非激活剂强刺激才使TOP/FOP值达到个位数。 3、有个基因，做了几次，发现TOP值没怎么升高，而FOP值降的挺厉害，导 … http://www.damor.cn/question/2173

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WebAs shown in figure 2, ENU-T1 cells showed lower activation of wnt signalling (TOPflash/FOPflash ratio 1,38 (σ: 0,47)) than 293T cells [46] which are known to have a low intrinsic wnt-activity... WebZoom Set of transfection grade T cell factor (TCF) reporter plasmids for use in TOPFlash and FOPFlash wnt/b-catenin activity assays. TCF Reporter Plasmid Kit MSDS (material …

Web24. mar 2024 · Our list of the best free Nintendo Switch games is here to help. We've rounded up the top free-to-play games on Nintendo Switch, from Switch Online … WebAs shown in figure 2, ENU-T1 cells showed lower activation of wnt signalling (TOPflash/FOPflash ratio 1,38 (σ: 0,47)) than 293T cells [46] which are known to have a …

http://www.biovector.net/cate/59.html WebFOPflash (mutant TCF binding sites) Transfection grade T cell factor (TCF) reporter plasmid containing 2 full & one incomplete copy of the Tcf binding site (mutated) followed by 3 …

Web16. mar 2024 · TOPflash/FOPflash luciferase assay Cells (5 × 10 3 ) were seeded into a 96‐well plate, and the next day, each well was transfected with a total of 50 ng DNA: 35 ng of KAR32 MSCV_C_HA_FLAG UBE2T expression plasmid or empty vector control, 10 ng of TOPflash or FOPflash, and 5 ng of β‐galactosidase plasmid (control for transfection ...

WebPlasmid M50 Super 8x TOPFlash from Dr. Randall Moon's lab contains the insert TCF/LEF binding sites and is published in Curr Biol. 2003 Apr 15. 13(8):680-5. This plasmid is available through Addgene. ... M51 Super 8x FOPFlash (TOPFlash mutant) human beta-catenin pcDNA3 pRL-SV40P ... prodigy psychiatric clinicWeb19. jan 2024 · A, B) Cells were transfected with TK-renilla and TOPflash or FOPflash in the presence of A) FLAG-DVl1 or C) GFP-FZD5 and HA-LRP6, or appropriate vector controls. A) 1-way ANOVA ( n = 9; F = 146.199, p < 0.001) followed by Bonferroni post-hoc analysis revealed increased DVL1-driven TOPflash activity in Lrrk2 knockout cells ( p < 0.001 … reinstall photoshop elements 19Web17. okt 2024 · Using TOPFlash/FOPFlash ratio to examine the effect of MYC on the activity of the WNT pathway, we found that DLD1 colon cancer cells transfected with TOPFlash reporter vector displayed constitutive TOPFlash activity that was reduced by treating cells with the inhibitor of the WNT pathway ICG-001 (Fig. 4c). reinstall photoshop elements 2022Web图1. 293a细胞分别转染TOPFlash和FOPFlash质粒24小时，再用20mM的氯化锂(LiCl)刺激24小时，最后用碧云天的萤光素酶报告酶基因检测试剂盒进行报告基因的检测。氯化锂是Wnt信号通路中GSK3β的抑制剂。 reinstall piawareWebCells were lysed and TOPflash and FOPflash activities were recorded in a luminometer. Both figures display the relative luciferase activity of cells transfected with TOPflash or FOPflash. B, after ... reinstall photoshop cs2Web17. okt 2024 · Using TOPFlash/FOPFlash ratio to examine the effect of MYC on the activity of the WNT pathway, we found that DLD1 colon cancer cells transfected with TOPFlash … prodigy public company limited ที่อยู่Web通常以TOP/FOP的比值表示。公式如下：转染有TOP-Flash和海肾荧光素载体的样本中采集得到的萤火虫荧光值F (Top)/采集得到的海肾荧光值R (Top) 转染有FOP-Flash和海肾荧光素载体的样本中采集得到的萤火虫荧光值F (Fop)/采集得到的海肾荧光值R (Fop) 本回答被提问者采纳 36 评论分享举报 1条折叠回答 2015-01-28 top/fop flash assay 比值一般为多大 2024 … prodigy property management wa